Alisa Karabegovic

• Independent qPCR COVID-19 diagnostics, ranging from sample preparation to analysis and manual report of results to patients
• Promoted to team leader, overseeing a small group dedicated to pre-analytical and analytical processes
• Independent organization of shift planning
• Responsible for distributing tasks among team members and ensuring analyses were completed within given timeframe
• Attentive hardcopy and electronic lab documentation

Thesis: PA1677, a new Crc Protein Interacting Partner involved in CCR?

• Performed cloning experiments with E. coli and utilized Pseudomonas aeruginosa as the model organism.
• Constructed translational and transcriptional plasmids, transforming them into E. coli, followed by Colony PCR.
• Coordinated sequencing of samples with an external company and analyzed the resulting data, constructing alignments using SnapGene.
• Executed electroporation and transformation of Pseudomonas aeruginosa.
• Conducted diauxie experiments and β-galactosidase assays.
• Undertook extensive research and maintained thorough documentation of laboratory work and results, which were analyzed and incorporated into my thesis.

• Sterile Handling of gingival mesenchymal stem cells
• Treatment with Zirconium and Titanium
• Gene Expression Analysis by PCR
• Microscopy Techniques
• Diligent Documentation of hardcopy records