Amol Ugale

Proficient, solutions focused researcher specializing in stem cell and cancer research, with a proven ability to develop innovative study concepts and secure funding through well-crafted grant proposals. Demonstrated expertise in project management and fostering team collaboration to advance scientific understanding. Strong communication and analytical skills have enhanced the effectiveness of research initiatives.

• Title: PhD
• Date of Birth: 20/01/88
• Nationality: Swedish

• 2014, American Society of Hematology, Travel award, 500 USD
• 2017, Vetenskapsrådet (Sweden), International Post-doc grant (for 3 years), 3150000 SEK

Cell / Stem cell biology

• Primary cells/ hematopoietic stem cells isolation and culture, Cancer cell lines/xenografts (patient derived cells) cultures, ES/iPS cells culture and maintainance, Small molecule library screening.

Cellular / Molecular analysis

• Gene overexpression / silencing (CRISPR-CAS9/shRNA Screens), viral transduction, transfection, cell-based assays for proliferation, migration, cytotoxic and metabolic studies.
• Flow cytometry (FACS): multicolour analysis and sorting, intracellular staining, magnetic cell separation.
• DNA, RNA and protein isolation, PCR, qRT-PCR, cloning, mutagenesis, Next generation sequencing (NGS) library preparation.
• Microscopy: Immunofluorescence and live cell imaging, confocal microscopy.
• Western blot, Cell surface capturing (CSC)-proteomics.

Animal work

• Transgenic and humanised murine model handling, Bone marrow isolation and in-vivo operations for functional assessment.

Software

• FlowJo, GraphPad Prism, Fiji/ImageJ, R programming

• 2021, Poster presentation at SY-Stem symposium, Vienna, Austria.
• 2016, Poster presentation at 58th Annual Meeting of American Society of Hematology, San Diego, California, USA.
• 2015, Poster presentation at 44th Annual Scientific Meeting of International Society for Experimental Hematology, Kyoto, Japan.
• 2014, Oral presentation at 56th Annual Meeting of American Society of Hematology (ASH), San Francisco, California, USA.
• 2014, Poster presentation at Stem Cell Niche Conference, Copenhagen, Denmark.

• 2019-2023, Supervised two Master students and one PhD student, University of Vienna
• 2018-2019, Supervised three Master students, City of Hope
• 2015-2017, Supervised three Master students, Research School Stem Cell Biology, Lund University
• 2012-2013, Laboratory Tutor, Biomedicine program, Lund University

• Ugale A, Shanmugam D, Pimpale LG, Rebhan E, Baccarini M., Signaling proteins involved in HSC fate determination are unequally segregated during asymmetric cell division., J Cell Biol, 06/01/24, 223, 9
• Yuan O, Ugale A, Marchi TD, Anthonydhason V, Konturek-Ciesla A, Wan H, Eldeeb M, Drabe C, Jassinskaja M, Hansson J, Hidalgo I, Velasco-Hernandez T, Cammenga J, Magee JA, Nimeus E and Bryder D., A somatic mutation in moesin drives progression into acute myeloid leukemia., Science Advances, 04/01/22, 8, 16
• Ugale A, Sawen P, Dudenhöffer-Pfeifer M, Wahlestedt M, Norddahl GL, Bryder D., MLL-ENL mediated leukemia initiation at the interface of lymphoid commitment., Oncogene, 06/01/17, 36, 22, 3207-3212
• Jaako P, Ugale A, Wahlestedt M, Velasco-Hernandez T, Cammenga J, Lindstörm MS, Bryder D., Induction of the 5S RNP_Mdm2-p53 ribosomal stress pathway delays the initiation but fails to eradicate established murine acute myeloid leukemia., Leukemia, 01/01/17, 31, 1, 213-221
• Lang S, Ugale A, Erlandsson E, Karlsson G, Bryder D, Soneji S., SCExV: a webtool for the analysis and visualization of single cell qRT-PCR data., BMC Bioinformatics, 10/01/15, 16, 320
• Ugale A, Norddahl GL, Wahlestedt M, Säwen P, Jaako P, Pronk CJ, Soneji S, Cammenga J and Bryder D., Hematopoietic stem cells are intrinsically protected against MLL-ENL mediated transformation., Cell Reports, 11/01/14, 9, 4, 1246-55
• Wahlestedt M, Norddahl GL, Sten G, Ugale A, Frisk MA, Mattsson R, Deierborg T, Sigvardsson M, Bryder D., An epigenetic component of hematopoietic stem cell aging amenable to reprogramming into a young state., Blood, 05/01/13, 212, 4257-64
• Norddahl GL, Pronk CJ, Wahlestedt M, Sten G, Nygren JM, Ugale A, Sigvardsson M, Bryder D., Accumulating mitochondrial DNA mutations drive premature hematopoietic aging phenotypes disrupt from physiological stem cell aging., Cell Stem Cell, 05/01/11, 8, 5, 499-510